16-alkylene pregnene derivatives



United States Patent This invention is concerned generally withnovel steroid compounds 'and with processes of preparing the same,

more particularly, it relates to 16-alkylene-11-oxygenated V steroids of the pregnane seriesunsatnrated in ring A and to novel processes for producing 16-alkylene steroids.

The new products of our invention may be represented by the following structural formula:

wherein R is alkylene, R is hydrogen'or acyl, R-"is hy droxy or oxygen, and X is hydrogen or halogen. 'The broken line between carbon atoms 1 and 2 indicates that a double bond may be present in this position.

The l6-alkylene steroids produced in accordance with the present invention possess extremely high anti-inflam matory activity, considerably greater than that ofthe' parent steroids, and are especially effective for the treatment of arthritis and related diseases since they can be administered for their cortisone-like action in low 'dosage thereby minimizing undesired side eifectsl In preparing our novel chemical compounds, the starting material utilized may be a 3-substituted-17a-hyclroxy- 1l-oxygenated-l6-alkylene-20Fpregnanoneor 3 substituted-17 u-hydroxy-l 1-oxygenated-l6 alkylene 2O allopregnanone which may be identified by the following structural formula:

ene-3a,l7a-dihydroxy-l1,20-pregnanedione which is repa:

resented by the following formula:

n? may is utilized as the starting material. However, it is clearto those skilled in the art that other starting materials, such as those described above, may be similarly converted to the desired and products.

It has been found that '16-a1kylene-3a,17a-dihydroxy- 11,20-pregnanedione may be caused to react with bromine in a chloroform medium to form 16-alkylene-Z1-bromo- 311,17 a-dihydroxy-l1,20-pregnanedione which has the following formula: a 10 V wherein R is as above.

Treatment of the above l6-alkylene-2l-brorno-3a,17adihydroxy-l1,20-pregnanedione with potassium iodide, potassium acetate and acetic acid in acetone yields 16- alkylene-Zm,l7a,2l-trihydroxy-l1,20- pregnanedione 21- 2 acetate which may be represented graphically as follows:

OHgOAc Oxidation of l6-alkylene-3a,l7u,21-trihydroxy-l1,20 pregnanedione ZI-acetate with sodium dichromate results in the formation of 16-alkylene-l7a,21-dihydroxy- 3,11,20-pregnanetrione Zl-acetate which has the following structural formula:

wherein R'is as above. I I

Treatment of 16-alkylene-l7a,2l dihydroxy 3,11,20-

pregnanetrione ZI-acetate with 1 mole of bromine yields 16-a1kylene-4-bromo-l 7a,2 1-dihydroxy-3, 1 1,20- preghane: trione which has the following structural formula:

CHzOAC 7 wherein R is as above.- 7

Reaction of the above 16-alkylene-4-bromo-17a-21 bromo-17u,2l-dihydroxy-3,11,20-pregnanetrione 21 -ace- Patented June 9, 1964- 3 tate with semicarbazide hydrohalide results in the formation of the B-sernicarbazone of 16-alkylene-17a,21-dihydroxy-4-pregnene-3,11,20-trione 2l-acetate which may be represented by the following formula:

CHzOAO wherein R is as above.

Upon reacting the latter semicarbazone of 16-alkylene- 17a,2l-dihydroxy-4-pregnene-3,11,20-trione with aqueous acetic acid-pyruvic acid there isformed 16-alkylene-17u, 2l-dihydroxy-4-pregnene-3, l1,20-trione 2l-acetate which has the following structure:

CHgOAc wherein R is as above.

The 21-acetate is hydrolyzed to the alcohol by treatment of 16-a1kylene-17a;21-dihydroxy-4-pregnene-3,11,

ZO-trione 21-acetate with potassium bicarbonate in aqueous methanol to yield l6-alky1ene-17a,2l-dihydroxy-4- pregnene-3,11,20-trione which may be represented by the following structural formula:

CH OH wherein R is as above. 1

The latter compound is then reacted with semicarbazide hydrochloride in methanol-dimethylformamide to form the 3,2Q-disemicarbazone of 16-alkylene-1 7a,21-dihydroxy-4-pregnene-3,11,20-trione which has the following structural formula:

CHQOH l C=NNHCONH1 wherein R is alkylene as above. v

Reduction of the 3,20-disemicarbazone of 16-alkylene- 17u,2l-dihydroxy-4-pregnene-3,11,20-trione with sodium boronhydride produces the 3,20-disemicarbazone of 16- 4 alkylene-l1p,1711,2l-trihydroxy-4-pregnene 3,20 dione,

which has the following formula:

wherein R is as above.

The 3,20-disemicarbazone of l6-alkylene-llfl,l7a,21- tri-hydroxy-4-pregnene-3,ZO-dione is reacted with'pymvic acid in aqueous acetic acid to remove the semicarbazone groups at the 3 and 20-positions to form 16-alkylene-11B, :,21-trihydroxy-4-pregnene-3,20-dione which may be chemically represented as follows:

CHgOH CHzOAG wherein R is as above.

Other 21-acylates such as the propionate, t-butyl acetate or benzoate may be prepared by reacting the 21-alcoho1 with the appropriate acyl anhydrides.

The l6-alkylene-l7a,2l-dihydroxy-3, l 1,20-pregnanetrione 2l-acetate may be reacted with 2 molecules of bromine to form 16-alkylene-2,4-dibromo-17a,2l-dihydroxypregnane-3,l1,20-trione 21-acetate which may be represented by the following formula:

CHzOAc wherein R is as above.

Treatment of the above compound with dimethyl aniline in dimethylformamide results in the desired l6-alkylene-17a,2l-dihydroxy-1,4-pregn'adiene-3,l1,20 trione- 21 acetate represented by the following formula:

CHrOAc n? e U wherein R is as above. The 21-acetate can be readily converted to the corresponding 21-o1-by hydrolysis, for example, in aqueous methanolic potassium bicarbonate to give l6-alkylene-l7a,2l-dihydroxy-1,4-pregnadiene-3, 11,20-trione represented by the following formula wherein R is as above.

wherein R is as above and X is hydrogen or fluorine and R" is hydroxy or oxygen. V

Treatment of these species with dimethyl aniline in dimethylformamide results in the desired l6-a1kylene-17a, 21 dihydroxy-l l-oxygenated-l,4-pregnadiene-3,20-dione 21-acetate and 16-alkylene-9a-fluoro-l7a,21-dihydr0xy- 11 oxygenated l,4-pregnadiene-3,ZO-dione 2l-acetate which may be represented graphically as follows:

CHzOAc wherein R, R" and X are as above. The species represented by the "above formula are highly potent anti-in flammatory agents.

The 21-acetate esters of these compounds can be hydrolyzed to the corresponding alcohols, 16-alkylene-17a, 21 dihydroxy-l l-oxygenated-1,4-pregnadiene-3,20-dione and 16-alkylene-9a-fluoro-17a,21-dihydroxy-1l-oxygenated-l,4-pregnadiene-3,20-dione represented by the following structural formula:

wherein R and R" are as above.

The l6-alkylene compounds described above may also be obtained from either the corresponding or alkyl compounds of the following formula:

CH2OH" in which R", X are as above and R"" is alkyl.

Treatment of this compound with semicarbazide base and semicarbazide hydrochloride leads to the corresponding 3,20-disemicarbazone of the following formula:

CHzOH v I =NNHCONHI I --OH W3 /g\/ d NHzCONHN-iv wherein R", X and R"" are as above.

The latter 3,20-disemicarbazone on heating in acetic acid and acetic anhydride is converted into the corresponding 16-unsaturated 3,20-disemicarbazone of the following formula:

onion wherein R, R", X and R"" are as above.

The latter lfi-unsaturated 'semicarbazone on treatment with hot aqueous acetic acid is converted to the .corresponding 3,20-dione of the following formula:

CHZOR wherein R, R", X and R"" are as above.

The latter compound on treatment with alkaline hydrogen peroxide or an alkyl hydroperoxide in the presence of a base or an organic peracid is converted into the correspondingl6a,17u-oxide of the following formula:

CHzOR' wherein R, R", X and R" are as above.

The above l6a,l7a-oxide on treatment with strong acid, for example, hydrogen bromide, hydrogen chloride, hydrogen fluoride or perchloric acid is converted into the corresponding 16-alkylene steroid of the following formula:

omoR' $0 i -OH Iv'fl R wherein R, R, R" and X are as above.

The 21-acetates may be converted to the corresponding 21-a1cohols of the following formula by acid or basic hydrolysis.

CHzOH 60 ly by means of Bacillus sphaericus.

The starting material for the present pr0ccss,vnamely, 3a,l7x-dihydr0xy-11-oxygenated-l6-alkylene 20 pregnanohe or the 3-acylate thereof can be prepared from the known 3a-hydroxy-16-pregnene-11,20-di0ne 3-acetate in accordance with the following procedure:

It has been found that 3a-hydroxy-l6-pregnene-l1,20- dione 3-acetate may be caused to react with diazoalkanes, such diazomethane and diazoethane to form 16a,l7aalkyleneazo-3oc-hydroxy-l1,20 pregnanedione 3-acetate which has the following structural formula:

wherein R is hydrogen or alkyl.

Upon heating 16a,l7oc-alkyleneazo-3a-hydroxy-11,20- pregnanedione 3-acetate, there is formed primarily l6- alkyl-3u-hydroxy-l6-pregnene-11,20-dione 3-acetate which may be represented by the following formula:

wherein R"" is alkyl as above.

Treatment of 16-alkyl-3a-hydroxy-l6-pregnene-11,20- dione 3-acetate, with an oxidizing agent such as hydrogen peroxide in the presence of sodium hydroxide or peracids such as peroxytrifiuoracetic acid results in the formation of 16,8-alkyl-16a,17a-epoxy-3a-hydroxy-l 1,20-pregnanedione which has the structural formula:

wherein R is as above.

Room temperature acetylation gives 16 3-alkyl-16a,l7aepoxy-3a-hydroxy-11,20-pregnanedione 3-acetate. Upon treatment with hydrogen bromide in acetic acid there is formed 16-alky1ene-3a,=l7a-dihydroxy 11,20 pregnanedione 3-acetate which may be represented as follows:

wherein R is as above.

Hydrolysis of this latter compound in aqueous methanolic potassium hydroxide leads to 16-alkylene-3a,17u-

dihydroxy-l1,20-pregnanedione represented by the following formula:

wherein R is as above.

The 16-alkylene steroids which possess extremely high anti-inflammatory activity may be administered orally, parenterally or topically. Thus, the active ingredient can be administered alone or may be associated with a carrier. A smaller quantity of the active 16-alkylene steroid may be administeredto the patient and have the same therapeutic effect as larger quantities of other steroids such as cortisone or hydrocortisone. Any of the carriers used in pharmaceutical practice may be employed Where there is no incompatibility with the active materials. The composition may take the form of tablets, powders, capsules, elixirs, syrups or other dosage forms which are particularly useful for oral ingestion. Liquid diluents are employed in sterile condition for parenteral use, namely, by injection. Such a medium may be a sterile solvent for the active material. For topical administration any of the known dermatologic vehicles may be employed. Such' vehicles for external application include water-soluble ointment bases, oils, petrolatum and jellies.

The following experimental part illustrates in detail some of the compounds whichconstitute this invention and methods for their production. However, this invention is not to be construed as limited thereby in spirit or in scope since it will be apparent to those skilled in the art that many modifications in materials and methods may be made without departing from the invention.

EXAMPLE 1 Preparation of 21-Br0m0-3u,17u-Dihydr0xy-16-Methylene-11,2.0-Pregnanedione 3-Acetate To a stirred solution of 209 mg. of 3a,17a-dihydroxyl6-methylene-11,20-pregnanedione 3-acetate (prepared as described hereinbelow) in 4 ml. of chloroform maintained at 40-45 C., is slowly added 85 mg. of bromine in 5 ml. of chloroform. Uptake of bromine is complete within 30 minutes. Ether is added and the mixture washed twice with cold 5% potassium bicarbonate solution. The organic extract is dried over magnesium sulfate and the colorless residue crystallized from acetone-ether gives colorless prisms of 21-bromo 3a,17a dihydroxy- 16-.

methylene-11,20-pregnanedione M.P. 195-200 dec.

Analysis.-Calculated for C H Q Br: C, 59.88; H, 6.91; Br, 16.60. Found: C, 60.50; H, 6.85; Br, 15.86.

The 3a,17u hydroxy 16 methylene 11,20 pregnanedione S-acetate used as a starting material in this example, is prepared starting with the known 3a-hydroxy- 16-pregnane-ll,20-dione B-acetate, in accordance with the following procedure: I

In a 500 ml. 3-neck flask equipped with condenser, dropping funnel and nitrogen inlet are placed 20 g. of potassium hydroxide in 9. 0 ml. of water, 100 ml. of methanol and 0 ml. of ether. A solution of 10 g. of N- methyl-N-m'trosotosylamide in 50 ml. of ether is placed in the dropping funnel.

Diazomethane is generated by warming the generation flask to 40-45 C. and cautiously adding the N-methyl- N-nitrosotosylamide-ether from the dropping funnel. Nitrogen is utilized to sweep the diazomethane into a solution of 20 g. of 3a-hydroxy-16-pregnene-11,20-dione 3-acetate in 100 ml. of tetrahydrofuran and 120 ml. ether. The process is continued until the steroid solution remains yellow for several hours. The product, Bot-hydroxy- 16ot,17a.-methylene-azo-11,20-pregnanedione S-acetate is largely precipitated from the reaction mixture. After :16 hours, the mixture is filtered, washed with ether and dried in arr.

37.4 grams, of 3ot-hydroxy-l6ot,17armethyleneazo-11,20- pregnanedione 3-acetate is placed in a 500 ml. roundbottom flask and heated by an oil bath in vacuo (pressure 0.6 mm). A manometer and 12 liter surge flask are in the line between the reaction flask and pump. trap. When the bath temperature reaches 180 C. the 3otehydroxyl6a,17a-methy1eneazo-11,2=0-pregnanedione 3-acetate begins to melt with evolution of nitrogen. The maximum pressure reached is 83 mm. After 10 minutes at 180- 182 C. the melt is' cooled. It has na E% 191 and is taken up in about 150 ml. of acetone, filtered through diatomaceous earth, concentrated to about 100 ml. and ether is slowly added to the boiling solution until crystallization occurs. These crystals of 3a-hydroxy-16-methyl-16-pregnene-11,20dione S-acetate have a melting point of l65168 C.

A solution of 20.0 g. of 3a-hydr0xy-16-methyl-16- Pregnene-ILZO-dione 3-acetate dissolved in 600 ml. of methanol, is cooled to 18 C., and 80 ml. of 30% hydrogen peroxide followed by 80 ml. of 2.5 N sodium hydroxide are added. Considerable material precipitates from solution, but all redissolves on stirring the reaction mixture at 25 -30 C. for 40 minutes. The solution is kept at 15-20 C. for 18 hours at which time the ultraviolet maximum at 249 has completely disappeared. Then 600 ml. of saturated salt water is slowly added, the crystalline precipitate is filtered, washed with water, and ried in air' and in vacuum. The 16a,17ot-epoxy-3et hydroxy-16fi-methyl-11,20-pregnanedione thus formed has a melting point of 176177 C.

Room temperature acetylation gives the 3a-hydroxy- 16a,17 x-epoxy-16fi-methyl-11,20-pregnanedione 3-acetate having a melting point of 162-164 C.

To a stirred solution of 5.05 g. of 3a.-hydroxy-16a,l7aepoxy-16,8-methyl-11,20-pregnanedione 3-acetate in 75 ml. of acetic acid maintained at 10-15 C. is added 25 ml. of cold 15% hydrogen bromide in acetic acid. After 35 minutes at 10-15 C. the mixture is concentrated to dryness in vacuo (temperature 15 C.) and the residue chromatographed on 200 g. of neutral alumina.

From the 50:50 petroleum ether-benzene to benzene eluates, there is obtained 1.60 g. 30:,17ot-dil1Y- droxy 16 methylene 11,20 pregnanedione 3-acetate; prisms from acetone-ether M.P. 198-200 C.

EXAMPLE 2 Preparation of 21-Bromo-3a,17a-Dihydr0xy-16-Methylene-11,20-Pregnanedi0ne To a stirred solution of 1.60 grams of 3a,17ot-dihydroxy-16-methylene-11,20-pregnanedione (prepared as described hereinbelow) in 32 ml. of chloroform maintained at 30-35 C. is slowly added 840 mg. of bromine in 48 ml. of chloroform. Uptake of bromine is complete 3 hours. Ether is added and the mixture washed twice with cold 5% potassium bicarbonate solution. The organic extract is dried over magnesium sulfate and the residue crystallized from acetone-ether to give 21 bromo 3u,17u dihydroxy 16 methylene- 11,20-pregnanedione. M.P. -198 dec.; [u] l34.

Analysis.-Calod. for C H O Br: C, 60.13; H, 7.11. Found: C, 60.31; H, 7.38.

The 3a,17a-dihydroxy-16-methylene-11,20-pregnanedione used as a starting material in this example is prepared starting with the 3a,17a-dihydroxy-16-methylene- 11,20-pregnanedione 3-acetate prepared as described in Example 1 in accordance with the following procedure:

To a stirred solution of 3.00 g. of 3u,17ot-dihydroxy- 16-methylene-11,20-pregnanedione 3-acetate in 90 ml. of

EXAMPLE 3 Preparation of 3a,]7a,21-Trihydr0xy-16-Methylene- 11,20-Pregnanedione 3,21-Diacetate 70 mg. of 21-brorno-3a,17a-dihydroxy-16-rnethylene- 11,20-pregnanedione 3-acetate, 55 mg. of potassium iodide and 70 mg. of anhydrous potassium acetate in 5 ml. acetone and 0.1 ml. acetic acid are refluxed 2 hours. The mixture is concentrated to dryness in vacuo and partitioned between ethyl acetate and water. The ethyl ace- O tate layer is washed with sodium chloride solution and dried over magnesium sulfate. Crystallization of the product from acetone-ether gives pure 3a,17a,21-trihydroxy-16-methylene-11,20-pregnanedione 3,2l-diacetate, M.P. 228-230, positive tetrazolium test.

I Analysis.-Calculated for C H O C, 67.80; H, 7.88. Found: C, 67.28; H, 7.75.

EXAMPLE 4 Preparation of 3a,] 7a,21-Trihydroxy-16-Methylene- 11,20-Pregnanedine 21-Acetate In the same manner as in Example 3, 21-bromo-3 (1,1706- dihydroxy-l6-methylene-11,20-pregnanedione (1.42 g.) (prepared as described in Example 2) is converted to 3 a,17a,21-trihydroxy-16-methyl-11,20-pregnanedione 21- acetate (1.28 g.), M.P. 172-175, [od +44.

Analysis.Calcd. for C H O C, 68.87; H, 8.18. Found: C, 68.47; H, 8.24.

EXAMPLE 5 Preparation of 1701,21-Dihydroxy-I6-Methylene-3,11,20- Pregnanetrione 21 -Acetate To a solution of 600 mg. of 3a,17a,21-trihydroxy-16- methylene-11,20-pregnanedione 21-acetate in 16 ml. of acetic acid is added 284 mg. of sodium dichromate in 13 ml. of acetic acid. After 3 hours at 25 C., 50% saturated aqueous sodium chloride solution is slowly added. The precipitated product is filtered, washed with water, dried in air and recrystallized from acetone-ether to give pure 17a,2l-dihydroxy-I6-methylene-3,11,20-pregnanetrione 21-acetate, M.P. 202-210".

EXAMPLE 6 Preparation of 4 -Br0m0-1 711,21 -Dihydr0xy-1 6-Methylene- 3,11,20-Pregnanetrione EXAMPLE 7 Preparation of fi-Semicarbazone of 17a,21-Dihydroxy-l6- Met/1ylene-4-Pregnene-3,1 1,20-Trione 21 -A cetate To 580 mg. of 4-bromo-1704,21-dihydroxy-16-methylene-3,11,20-pregnanetrione 21-acetate in 20 ml. of acetonitrile under nitrogen is added a slurry of 300 mg. of vsemicarbazide hydrochloride and 200 mg. sodium bicar- 12 bonate in 4 ml. of Water. After 2 hours, the acctonitrile is removed in vacuo, water added and the crystalline 3-semicarbazone of 17a,21-dihydroxy 16 methylene-4- pregnene-3,11,20-trione 21-acetate is filtered, washed with Water, and dried in air.

EXAMPLE 8 Preparation of 170;,21-Dihydroxy-I6-Methylene-4Pregnene-3,11,20-Tri0ne ZZ-Acetate (16-Methylene Cortisone Acetate) 500 mg. of the semicarbazone of 17a,21-dihydroxy-16- methylene-4-pregnene-3,l1,20-trione is dissolved in ml. of acetic acid, 1.5 ml. of pyruvic acid and 5 ml. of water. After 18 hours at C., water is added and the mixture is extracted with chloroform. The chloroform extract is washed with aqueous potassium bicarbonate, water, and dried over sodium sulfate. Removal of the solvent gives crude 17a,21-dihydr0xy-16-methylene 4 pregnene-3,11, 20-trione 21-acetate, which is purified by crystallization from acetone-ether.

EXAMPLE 9 Preparation 0 1 7a,21 -Dihyar0xy-16-MethyIene-4-Pregnene-3,11,20-Trio1ze (16-Methylene Cortisone) This compound is obtained by treatment of 500 mg. of the corresponding 2l-acetate (the product of Example 8) in 15 ml. of methanol with 500 mg. of potassium bicarbonate in 5 ml. of Water under nitrogen at reflux for 10 minutes. The mixture is cooled, neutralized with 0.5 ml. of acetic acid in 5 ml. of water, the methanol removed in vacuo and the product extracted into ethyl acetate. Concentration of the ethyl acetate gives crystalline 17a,21-dihydroxy-16-methylene-4-pregnene-3,1 1,20- trione.

EXAMPLE 10 I Preparation of 3,20-Disemicarbaz0ne of ]7a,21-Dihydroxy-J6-Methylene-4-Pregnene-3,11,20-Tri0ne To a stirred solution of 500 mg. of 17a,21-dihydroxy- 16-methylene-4-pregnene-3,11,20-trione in 12.5 ml. of methanol and 3 ml. of dimethylformamide kept under nitrogen is added a slurry of 680 mg. of semicarbazide hydrochloride and 370 mg. of sodium bicarbonate in 1 ml. of water. The stirred mixture is refluxed 3 /2 hours and maintained at 45 C. for 17 hours. It is then cooled to 20 C. and 50 ml. of 50% saturated aqueous sodium chloride is added. After 2 hours at 0 C. the precipitate of the 3,20-disemicarbazone of 17a,21-dihydroxy-16- methylene-4-pregnene-3,11,20-trione 21-acetate is filtered, washed with water until free of chloride ion and dried in an.

EXAMPLE 11 Preparation of the 3,20-Disemicarbazone of 115,] 711,21-

Trihydroxy-I G-Methylelze-4-Pregnene-3,20-Dione To a stirred solution of 600 mg. of the 3,20-disemicarbazone of 17a,21-dihydroxy-16-methylene-4-pregnene- 3,11,20-trione 21-acetate in ml. of tetrahydrofuran and 11 ml. of Water under nitrogen is added 200 mg. powdered sodium borohydride. The stirred suspension is refluxed minutes and then cooled to 15 C. Aqueous acetic acid (3 ml. of 30%) is added cautiously and most of the tetrahydrofuran is removed in vacuum. Addition of 5 ml. of methanol and 5 ml. of water induces the product to crystallize. Following addition of 10 ml. of a saturated sodium chloride solution and aging at 0 C. the product 3,20-disemicarbazone of 11,13,17a,21-trihydroxy 16 methylene 4 pregnene-3,20-dione is filtered, washed with Water, and dried in air.

EXAMPLE 12 Preparation of 1 1 [3,1 7 a,21 -Trihydr0xy-1 6 -M ethyl ene-4 Pregnene-3,20-Dione (16-Methylene H ydrocorlisone) To a solution of 510 mg. of the 3,20-disemicarbazone EXAMPLE 13 Preparation of J 8,1 7a,21 -Trihydrxy-1 6-Methylene-4- Pregnene-3,20-Di0ne 21 -Acetate (1 6-M ethylene H ydrocortisone Acetate) The product of the previous Example 12 is acetylated at C.21 as follows: A solution of 100 mg. of l1,8,17a,- 21 trihydroxy 16- methylene 4 pregnene 3,20- dione in 1.0 ml. of pyridine and 0.5 ml. of acetic anhy dride is prepared. After 18 hours at 25 C., the solution is taken to dryness in vacuo and the solid residue purified by crystallization in acetone-ether to give 11fi,17a,21-trihydroXy-16-methylene-4-pregnene-3,20-dione 21-acetate.

EXAMPLE 14 Preparation of 1 711,21-Dihydr0xy-l6-Methylene-1,4- Pregnaa'iene-3,11,20-Tri0ne Treatment of 17a,21-dihydroXy-16-methylene-4-pregnene-3,11,20-trione prepared as in Example- 9 or the corresponding 21-acetate preparedas in Example 8 with Bacillus sphaericus under suitable conditions leads to 1704,21 dihydroxy 16 methylene 1,4 pregnadienc- 3,11,20-trione.

EXAMPLE 15 Preparation of 2,4-Dz'brom0-1 7a,21-Dihydr0xy-l6- Methylene-3,11,ZO-Pregnanetrione 21 -Acetate To a stirred solution of 200 mg. of flail-dihydroxy- 16-methylene-3,11,20-pregnanetrione 2l-acetate (prepared as described in Example 5) in 4 ml. of chloroform and 0.05 ml. of acetic acid maintained at -15 C. is added dropwise one-half of a solution of 162 mg. of bromine in 0.47 ml. of chloroform and 0.53 ml. of acetic acid. The mixture is warmed to 0 C. and the remainder of the bromine added. Sodium acetate (0.1 g.) in 0.4 ml. of Water is added followed by a few drops of 5% aqueous sodium sulfite. Additional chloroform and water are added andv the mixture is extracted with chloroform. The chloroform layer" is washed with aqueous potassium bicarbonate, aqueous sodium chloride and dried over magnesium sulfate. Evaporation of the solvent leaves as a residue 275 mg. of 2,4-dibromo-17a,21-dihydroxy 16 methylene 3,11,20 pregnanetrione 21 acetate.

EXAMPLE 16 Preparation of 170:,21-Dihydroxy-16-Methylane-1,4- Pregnadiene-3J1,20-Tri0ne 21-Acetate To a solution under nitrogen of 2.75 mg. of the 2,4-dibromo 17a,21 dihydroxy 16 methylene 3,11,20- pregnanetrione 21-acetate in 3.3 ml. dimethyl formamide .is added 53 mg. of sodium bromide. After 1 hour at 25 C. 0.66 ml. of dimethyl aniline is added and the mixture maintained at 135 C. for 2% hours. The mixture is cooled, added dropwise to dilute hydrochloric acid, and solid, crude product filtered washed with dilute hydrochloric acid, water and dried in air. Treatment with charcoal, followed by chromatography on neutral alumina gives diene-3,l1,20-trione 21-acetate M.P. 214217 C.;

Analysis calculated for C H O .C, 69.88; H, 6.84. Found: C, 69.96; H, 6.94.

17 a,2l-dihydroxy-16-methylene-1,4-pregna- 14 EXAMPLE 17 Preparation of 17u,21-Dihydr0xy-16-Methylene-1,4- Pregnadiene-3,1 1,20-Tri0ne This compound is obtained by treatment of 1.0 g. of 17u,21 dihydroxy 16 methylene 1,4 pregnadiene- -3,1l,20 trione 21-acetate in 30 ml. of methanol with 1 g. 'of potassium bicarbonate in 10 ml. of water under nitrogen at reflux temperature for. 7 minutes. The mixture is cooled, neutralized with 1 ml. of acetic acid in 10 ml. of Water, the methanol removed in vacuo and the product is extracted into ethyl acetate. Removal of the ethyl acetate' gives 17a,2l-dihydroXy-16 methylene-1,4-pregnadime-3,1 1,20-trione.

EXAMPLE 18 Preparation of 11,8,17a,21-Trihydr0xy-16-Methylene- 1,4-Pregliadiene-3,20-Dione 21 Acetate The 1,2-dehydrogenation of 11B,l7a,21-trihydroxy-16- methylene-4-pregnene-3,20-dione 21-acetate prepared as in Example 13 may be carried out microbiologically by means of Bacillus sphaericus to yield 1lB,17a,21-trihydroxy-16-methylene-1,4-pregnadiene-3,20-dione, which on acetylation with acetic anhydride-pyridine gives 11,43,170,- 21 trihydroxy 16 methylene 1,4 pregnadiene 3,20- .dione 21-acetate. g

EXAMPLE 19 Preparation of the 3,20-Disemicarbaz0ne of 9a-Flu0r0 9 11,8,17oc,21 Trihydroxy 16a Methyl J ,4 Pregnadiene-3,20-Di0nc rgggP 292 my. Em. 25,500; 240 my Em. 21,400

In similar manner are prepared the 3,20-disemicarbazone of 9a-fluoro-llfi,17a,2l-trihydroxy-16fi-methyl-1,4-

pregnadiene-lZO-dione, 3,20-disemicarbazone of 115, 1704,21 trihydroxy 16a methyl 1,4 pregnadiene- 3,20-dione; 3,20-disemicarbazone of 1lB,17a,21-trihydroxy 16B methyl-- 1,4 pregnadiene 3,20 dione; 3,20-disemicarbazone of 17u,21-dihydroxy-16u-methyl- 1,4 pregnadiene 3,11,20 trione; 3,20 disemicarbazone of 170:,21 dihydroxy 16B methyl 1,4 pregnadiene- 3,11,20 trione; 3,20-disemicarbazone of 9a-flu0ro-1IB, 1704,21 trihydroxy 16a methyl 4 pregnene 3,20- dione; 3,20-disemicarbazone of 9a-fluo1'o-11B,17u,21-tri- .hyrdroxy 16B methyl 4 pregnene 3,20 dione; .3,20-disemicarbazone of 11 5*,17a,21 trihydroxy cmethyl-4-pregnene-3,20-dione; 3,20-disemicarbazone of 11,3,17oc, 21 trihydroxy methyl 4 pregnene- 3,20-dione; 3,20-disemicarbazone of 17a,21-dihydroxy- 16a-methyl-4-pregnene-3J1,20 trione and 3,20-disemicarbazone of l7a,2l-dihydroxy-16fl-methyl-4-pregnene- .3,11,2O-trione.

EXAMPLE 20 Preparation of 9a-Flu0r0-11p,21-Dihydr0xy-16-Methyl- J ,4,16-Prepgatriene-3,20-Dione 21 -A cetate A solution of 500 mg. of the 3,20-disemicarba'zone of pregnadiene-3,20-dione in 10 ml. of acetic acid and 0.5

m1. acetic anhydride is refluxed under nitrogen for one hour to produce the corresponding 3,20-disemicarbazone of 115,21 dihydroxy 16 methyl 1,4,16 pregnatriene-3,20-dione ZI-acetate. The reaction mixture is cooled, 13 m1. of water is added and the mixture heated ammonium hydroxide. perature, water is added and the mixture thoroughly exresidue chromatographed on neutral alumina.

on the steam bath for 5 hours. It is then concentrated in vacuo nearly to dryness and water and chloroform added. The mixture is thoroughly extracted with chloro form, and the chloroform extract washed with excess aqueous potassium bicarbonate, saturated salt solution and dried over magnesium sulfate. Chromatography of the residue on neutral alumina and crystallization of pertinent benzene-chloroform fractions gives 9a-fluoro- 113,21 dihydroxy 16 methyl 1,4,16 pregnatriene- 3,20-dione 21-acetate; M.P. 228233;

RX? 243 m Em. 22,000

. Similar treatment of the 3,20-disemicarbazone of 9afluoro 11,8,17a,21 trihydroxy 16B methyl 1,4 pregnadiene-3,20-dione also leads to 9a-fluoro-1l/3,21-dihydroxy 16 methyl 1,4,16 pregnatriene 3,20 dione 2l-acetate.

In a similar manner 11,8,2l-dihydroxy-l6-rnethyl-1,4, 16-pregnatriene-3,20-dione 21-acetate; 21-hydroxy 16- methyl 1,4,16 pregnatriene 3,11,20 trione 21- acetate; 90c fluoro 11,8,21 dihydroxy 16 methyl-4,16- pregnadiene-3,20-dione 21-acetate; 115,21-dihydroxy-16- methyl-4,16-pregnadiene-3,20-dione 2l-acetate; and 21- hydroxy 16 methyl 4,16 pregnadiene 3,11,20 trione 21-acetate are obtained.

EXAMPLE 21 Preparation of 9a-FIu0r0-1][3,21-Dihydr0xy-1(iii-Methyl- 1 6 01,1 7a-Oxid0-l ,4-Preglzadiene-3,20-Dine 21-A cetate To a stirred solution of 500 mg. of 9a-fluoro-11B,21- dihydroxy 16 methyl 1,4,16 pregnatriene 3,20- dione 21-acetate in ml. of benzene and 5 ml. of chloroform are added 0.50 ml. of t-butyl hydroperoxide and 0.1 ml. of a 35% methanolic solution of benzyl-trimethyl After 18 hours at room temtracted with chloroform. The chloroform extract is washed with saturated aqueous sodium chloride'and dried over magnesium sulfate. Evaporation of the solvent and crystallization of the residue from acetone-ether gives 90: fluoro 115,21 dihydroxy 16B methyl 160:,1711- oxido-1,4-pregnadiene-3,20-dione 21-acetate.

In a similar manner are prepared 115,21-dihydroxy- 165 methyl 16a,17a oxido 1,4 pregnadiene -3,20-

dione 21-acetate; 21-hydroxy-l6,B-methyl-l6a,l7a-oxido- 1,4-pregnadiene-3,11-20-trione 21-acetate; 9a-fluoro-11fl, 21 dihydroxy 16B methyl 16oz,l7ot oxido 4 pregnene-3,20-dione 21-acetate; 11,8,21-dihydroxy-16fl-methyl- 16a,17a-oxido-4-pregnene-3,20-dione 2l-acetate, and 21- hydroxy 16,8 methyl 16a,17a oxido 4 pregnene- 3,11,20-trione 21-acetate.

EXAMPLE 22 To a stirred solution of 600 mg. of 9a-fluoro-1l5,21-

dihydroxy-l6B-methyl 16a,17a oxido 1,4 pregnadiene-3,20-dione 21-acetate in 10 ml. of acetic acid maintained at 1015 C. is added 3 ml. of cold 10% hydrogen bromide in acetic acid. After minutes the mixture is concentrated to dryness in vacuo (temp. 15) and the Combination of pertinent benzene-chloroform fractions and crystallization leads to the desired 9a-fluoro-11,6,17u,21-trihydroxy-16-methylene 1,4 -pregnadiene 3,20 dione 21- acetate.

finoro 11,6,21 dihydroxy 16/3 methyl 16a,17aoxido-1,4-pregnadiene-3,20-dione 21-acetate in 2.4 ml. of tetrahydrofuran is added 3.5 ml. of a 2:1 by weight mixture of hydrogen fluoride in tetrahydrofuran. After 2 hours at 15 the reaction mixture is pipetted slowly into excess cold aqueous sodium carbonate solution. The mixture is extracted with chloroform and the chloroform extract washed with water and saturated aqueous sodium chloride. Chromatography of the residue on neutral alumina as above also leads to the desired fluoro 11,8,17a,21 trihydroxy 16 methylene 1,4- pregnadiene-B,20-dione-21-acetate.

EXAMPLE 23 Preparation of 9ci-Flu0ro-11,8,17a,21-Trihydroxy-16- M ethylene-1 ,4 -Pregnadiene-3 ,2 O-Dione This compound is obtained by treatment of 200 mg. of the corresponding 21-acetate (the product of Example 22) in 10 ml. of methanol with 200 mg. of potassium bicarbonate in 3 ml. of water under nitrogen at reflux for 10 minutes. The mixture is cooled, neutralized with 0.3 ml. of acetic acid in 5 ml. of water, the methanol removed in vacuo and the product extracted into ethyl acetate. Concentration of the ethyl acetate gives crystalline 9afluoro-l1fi,17a,2l-trihydroxy 16 methylene 1,4 pregnadiene-3,20-dione.

In a similar manner may be obtained llB,l7a,2l-trihydroxy-16-methylene 1,4 pregnadiene-3,20-dione; 17a, 21-dihydroxy 16 methylene 1,4 pregnadiene-3,11,20- trione; 9a-flu0ro-11fl,17a,21-trihydroxy 16 methylene- 4-pregnene-3,20-dione; 11B,17 x,2l-trihydroxy 16 methylene-4-pregnene 3,20 dione and 17a,21-dihydroxy-16- methylene-4-pregnene-3,11,20-trione.

EXAMPLE 24 Preparation of 9a-Fluoro-1 118,] 711,21 -Trihydr0xy-16- M ethylene-1 ,4-Pregnadiene-3 ,2 O-Dione mg. of 9a-fluoro-11B,17a,21-trihydroxy-16-methylene-4-pregnene-3,20-dione 2l-acetate is prepared as described in Example 22, and is treated with Bacillus sphaericus to produce the corresponding 9oc-flt10I'O-1lj3, 17a,2l-trihydroxy-16-methylene 1,4 pregnadiene-3,20- dione.

EXAMPLE 25 Preparation of 9a-Flaoro J1B,17a,21 Trihydr0xy-16- Methylane-1,4-Pregnadiene-3,20-Dione 21 -t-Butyl-A celate To a stirred solution of 50 mg. of 9a-fiuoro-1lfi,17a, 21-trihydroxy-16-methylene 1,4 pregnadiene-3,20-dione (the product of the previous example) in 0.5 ml. of pyridine at 10 is added 0.3 ml. of t-butylacetyl chloride. After two hours at 25 water is added and the precipitated product filtered, washed with dilute hydrochloric acid, dilute sodium bicarbonate solution, water and dried in air.

In a similar manner may be obtained the 21-t-butyl acetates of 1lfl,17a,21-trihydroxy-16-methylene-1,4-pregnadiene-3,20-dione; 17a,21-dihydroxy 16 methylene-1,4- pregnadiene 3,11,20 trione; 9a-fluoro-11fl,17a,21-trihydroxy-16-methylene-4-pregnene 3,20 dione; 11,8,17a, 21-trihydroxy-16-methylene-4-pregnene 3,20 dione and 17a,2l-dihydroxy-l6-methylene 4 pregnene-3,11,20-trione.

In an analogous manner may be prepared the corresponding 2l-propionates 21-benzoates and other 21-esters of aliphatic and aromatic acids.

ylene-4-pregnene-3,20-dione 21-acetate is prepared as described in Example 22, and is treated with Bacillus 1? sphaericus to produce the corresponding 9a-fiuoro-11fi, 17u,21-trihydroXy-16-methylene 1,4 pregnadiene-3,20- dione.

EXAMPLE 25 Preparation 9a-Fluoro 1113171121 Trihydroxy-16- M ethylene-1 ,4-Pregnadiene-3,20-Di0ne 21 -t-B utyl-A celate To a stirred solution of 50 mg. of 9c:fi110lO-11B,17oz, 2l-trihydroxy-l6-methylene 1,4 pregnadiene-3,20-dione (the product of the previous example) in 0.5 ml. of pyridine at is added 0.3 ml. of t-butylacetyl chloride. After two hours at 25 water is added and the precipitated product filtered, washed with dilute hydrochloric acid, dilute sodium bicarbonate solution, water and dried 1n air.

In a similar manner may be obtained the 21-t-butyl acetates of 11/3,17ot,2l-trihydroxy-l6-methylene-1,4-pregnadiene-3,20-dione; 1701,2l-dihydroxy-16-methylene-1,4- pregnadiene 3,11,20 trione; 9u-fluoro-11,B,17ot,21-trihydroxy-l6-methylene-4-pregnene 3,20 dione; 11B,l7a,21- trihydroxy-16-methylene-4-pregnene-3,ZO-dione and 170a, 2 l-dihydroxy-16-methylene-4-pregnene-3, 1 1,20-trioue.

In an analogous manner may be prepared thecorrespending 21-propionates ZI-benzoates and other 21-esters of aliphatic and aromatic acids.

EXAMPLE 26 Preparation of 9oa-Fluoro-1 7a,21-Dihydr0xy-1 6-Me thylene-l,4-Pregnadiene il1,20-Trims 21 -A cetate To'a stirred solution of 120 mg. of 9oc-fl11OIO-11 3,17a, 2l-trihydroxy-l6-methylene 1,4 pregnadiene-3,20-dione 21-acetate in 3 m1. of glacial acetic acid is added 43 mg. of sodium dichromate in 2 ml. of glacial acetic acid at 20 C. The mixture is stirred at 2025 for 3 hours, diluted with water and extracted with chloroform. The chloroform extract is Washed with aqueous potassium bicarbonate solution, saturated sodium chloride solution, dried over magnesium sulfate and taken to dryness.

its Crystallization of the residue from acetone-ether gives 9a-fluoro-17a,21-dihydroxy-l6-methylene 1,4 pregnadiene-3,11,20-trione 21-acetate. The corresponding 21- alcohol is obtained by treatment of the 21-acetate with aqueous methanolic potassium bicarbonate as described in Example 17.

In a similar manner 9 -fluoro-17a,21-dihydroxy-16- methylene-4-pregnene-3,11,20-trione ZI-acetate and 21- alcohol are obtained from 9ot-flu0ro-1lfi,17a,21-trihydroxy-16-methylene-4-pregnene-3,20-dione 21-acetate.

Various changes and modifications may be made in the present invention, certain preferred embodiments of which are herein disclosed, without departing from the scope thereof; to the extent that these changes and modifications are within the scope of the appended claim, they are to be considered a part of this invention.

We claim:

9ot-fluoro-11,8,21-dihydroxy 16/3 methyl-l6oc,l7oa-oxido-l,4-pregnadiene6,20-dione 21-acetate.

References Cited in the file of this patent UNITED STATES PATENTS 2,666,016 Hechter et al. Jan. 12, 1954 2,756,179 Fried et al July 24, 1956 2,863,862 Djerassi et al. Dec. 9, 1958 2,865,808 Agnello et al. Dec. 23, 1958 2,867,630 Pederson et al. Jan. 6, 1959 2,867,633 Lincoln et al. Jan. 6, 1959 2,932,639 Oliveto et al. Apr. 12, 1960 2,932,656 Day Apr. 12, 1960 2,942,012 Taub et al June 21, 1960 2,944,070 Kollonitsch et al. July 5, 1960 2,954,386 Beyler Sept. 27, 1960 2,966,504 Taub et al. Dec. 27, 1960 OTHER REFERENCES Meystre et al.: Helv. Chim. Acta., vol. 39, pages 734-42 (1956). 

